Article
Ypt1 and TRAPP interactions: optimization of multicolor bimolecular fluorescence complementation in yeast.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Lipatova Zhanna, Kim Jane J, Segev Nava
Abstract excerpt
Ypt/Rab GTPases are conserved molecular switches that regulate the multiple vesicular transport steps of all intracellular trafficking pathways. They are stimulated by guanine-nucleotide exchange factors (GEFs). In yeast, Ypt1 regulates transport from the endoplasmic reticulum (ER) to two alternative pathways: secretion and autophagy. Ypt1 is activated by TRAPP, a modular multi-subunit GEF. Whereas TRAPP I...
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