Article
New Gateway-compatible vectors for a high-throughput protein-protein interaction analysis by a bimolecular fluorescence complementation (BiFC) assay in plants and their application to a plant clathrin structure analysis.
Bioscience, biotechnology, and biochemistry - 1 Jan 2015
Nishimura Kohji, Ishikawa Syouta, Matsunami Erika, Yamauchi Junji, Homma Keiichi, Faulkner Christine, Oparka Karl, Jisaka Mitsuo, Nagaya Tsutomu, Yokota Kazushige, Nakagawa Tsuyoshi
Abstract excerpt
Protein-protein interactions (PPI) play key roles in various biological processes. The bimolecular fluorescence complementation (BiFC) assay is an excellent tool for routine PPI analyses in living cells. We developed new Gateway vectors for a high-throughput BiFC analysis of plants, adopting a monomeric Venus split just after the tenth β-strand, and analyzed the interaction between Arabidopsis thaliana coated...
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