Article

The Crystal Structure of Engineered Nitroreductase NTR 2.0 and Impact of F70A and F108Y Mutations on Substrate Specificity

2023-03-17

Abstract excerpt

Bacterial nitroreductase enzymes that convert prodrugs to cytotoxins are valuable tools for creating transgenic targeted ablation models to study cellular function and cell-specific regeneration paradigms. We recently engineered a nitroreductase (“NTR 2.0”) for substantially enhanced reduction of the prodrug metronidazole, which permits faster cell ablation kinetics, cleaner interrogations of cell function, ablati...

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Literature Corpus work: 995dc834-aea9-54fc-810a-2c36f94c79d2
DOI: 10.20944/preprints202303.0315.v1

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