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Improved Protocol for Single-Nucleus RNA-sequencing of Frozen Human Bladder Tumor Biopsies

2022-10-14

Abstract excerpt

This paper provides a laboratory workflow for single-nucleus RNA-sequencing (snRNA-seq) including a protocol for gentle nuclei isolation from fresh frozen tumor biopsies, making it possible to analyze biobanked material. To develop this protocol, we used non-frozen and frozen human bladder tumors and cell lines. We tested different lysis buffers (IgePal and Nuclei EZ) and incubation times in combination with diffe...

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Identifiers and source

Literature Corpus work
9580f242-274d-5adc-b8cc-1cc39bcf4927
DOI
10.1101/2022.10.14.512220
Open publication

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Improved Protocol for Single-Nucleus RNA-sequencing of Frozen Human Bladder Tumor BiopsiesDOI 10.1101/2022.10.14.512220
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