Article

Characterization and Optimization of Multiomic Single-Cell Epigenomic Profiling

2023-05-08

Abstract excerpt

The snATAC + snRNA platform allows epigenomic profiling of open chromatin and gene expression with single-cell resolution. The most critical assay step is to isolate high-quality nuclei to proceed with droplet-base single nuclei isolation and barcoding. With the increasing popularity of multiomic profiling in various fields, there is a need for optimized and reliable nuclei isolation methods, mainly for human tiss...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e19a7fb5-f751-5097-8a79-f3ed3698c06c
DOI: 10.20944/preprints202305.0530.v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Characterization and Optimization of Multiomic Single-Cell Epigenomic ProfilingDOI 10.20944/preprints202305.0530.v1Read

A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumorsSemantic proximity Read

A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumorsSemantic proximity Read

Single-Nucleus RNA-Seq: Open the Era of Great Navigation for FFPE TissueSemantic proximity Read

Improved Protocol for Single-Nucleus RNA-sequencing of Frozen Human Bladder Tumor BiopsiesSemantic proximity Read

Single-cell and single-nucleus RNA-sequencing from paired normal-adenocarcinoma lung samples provide both common and discordant biological insightsSemantic proximity Read

Benchmarking Single-Cell RNA Sequencing Protocols for Cell Atlas ProjectsSemantic proximity Read

The challenges of single cell transcriptomics on difficult human tissue: the placentaSemantic proximity Read

Advancing Nuclei Isolation from Frozen Human Heart for Single-Nucleus RNA Sequencing ApplicationsSemantic proximity Read

Comparative Analysis of Single-Nucleus and Single-Cell RNA Sequencing in Human Bone Marrow Mononuclear Cells: Methodological Insights and Trade-offsSemantic proximity Read

Cryopreservation of human cancers conserves tumour heterogeneity for single-cell multi-omics analysisSemantic proximity Read

Single‐cell RNA‐sequencing of retrieved human oocytes and eggs in clinical practice and for human ovarian cell atlasingSemantic proximity Read

Optimized Methods for Obtaining scRNA-seq and snRNA-seq Data from Skeletal Muscle Stored in Nucleic Acid Stabilizing PreservativeSemantic proximity Read