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A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumors

1 May 2020

Abstract excerpt

Single-cell genomics is essential to chart tumor ecosystems. Although single-cell RNA-Seq (scRNA-Seq) profiles RNA from cells dissociated from fresh tumors, single-nucleus RNA-Seq (snRNA-Seq) is needed to profile frozen or hard-to-dissociate tumors. Each requires customization to different tissue and tumor types, posing a barrier to adoption. Here, we have developed a systematic toolbox for profiling fresh and...

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PMID: 32405060
DOI: 10.1038/s41591-020-0844-1

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