Article

Amplification-free Library Preparation Improves Quality of Hi-C Analysis

2019-02-27

Abstract excerpt

PCR amplification of Hi-C libraries introduces unusable duplicates and results in a biased representation of chromatin interactions. We present a simplified, fast, and economically efficient Hi-C library preparation procedure that generates sufficient non-amplified ligation products for deep sequencing from 30 million Drosophila cells. Comprehensive analysis of the resulting data indicates that amplification-free...

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Identifiers and source

Literature Corpus work: 9242caea-7353-5a2d-98f9-89b459ace4dd
DOI: 10.1101/562280

Open publication

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