Article

PU.1-driven enrichment enables microglia profiling from frozen brain tissue using the high-throughput Smart-seq3xpress method

2025-08-15

Abstract excerpt

<h4>ABSTRACT</h4> Single-cell transcriptomics has revealed the central role of microglia in brain development, homeostasis, and disease, particularly in the context of neuroinflammation. While single-cell RNA-sequencing enables targeted microglial analysis from fresh tissue, studying these cells in cryopreserved or archival samples remains challenging due to the lack of protocols for their specific enrichment. We...

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Literature Corpus work: 91db80c3-e275-5610-96fb-4140da3f083a
DOI: 10.1101/2025.08.11.669607

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