Article

Establishment of a simplified preparation method for single-nucleus RNA-sequencing and its application to long-term frozen tumor tissues

2020-10-23

Abstract excerpt

Recent advances allowing the genomic analysis of individual cells from a bulk population have provided intriguing new insights into areas such as developmental processes and tumor heterogeneity. Most approaches to date, however, rely on the availability of fresh surgical specimens, thereby dramatically reducing the ability to profile particularly rare tissue types. Pediatric central nervous system tumors – the lea...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0e6bb039-a78e-5f42-bf27-7245206ad065
DOI: 10.1101/2020.10.23.351809

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Establishment of a simplified preparation method for single-nucleus RNA-sequencing and its application to long-term frozen tumor tissuesDOI 10.1101/2020.10.23.351809Read

A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumorsSemantic proximity Read

A single-cell and single-nucleus RNA-Seq toolbox for fresh and frozen human tumorsSemantic proximity Read

Single-Nucleus RNA-Seq: Open the Era of Great Navigation for FFPE TissueSemantic proximity Read

Tumor type and cell type-specific gene expression alterations in diverse pediatric central nervous system tumors identified using single nuclei RNA-seqSemantic proximity Read

Probing glioblastoma and its microenvironment using single-nucleus and single-cell sequencingSemantic proximity Read

Identifying tumor type and cell type-specific gene expression alterations in pediatric central nervous system tumorsSemantic proximity Read

snCED-seq: High-fidelity cryogenic enzymatic dissociation of nuclei for single-nucleus RNA-seq of FFPE tissuesSemantic proximity Read

scONE-seq: A one-tube single-cell multi-omics method enables simultaneous dissection of molecular phenotype and genotype heterogeneity from frozen tumorsSemantic proximity Read

snCED-seq: High-fidelity cryogenic enzymatic dissociation of nuclei for single-nucleus RNA-seq of FFPE tissuesSemantic proximity Read

Extraction of nuclei from archived post-mortem tissues for single-nucleus sequencing applicationsSemantic proximity Read

PU.1-driven enrichment enables microglia profiling from frozen brain tissue using the high-throughput Smart-seq3xpress methodSemantic proximity Read

The Single-Cell Pediatric Cancer Atlas: Data portal and open-source tools for single-cell transcriptomics of pediatric tumorsSemantic proximity Read