Article
Alzheimer's Patient Microglia Exhibit Enhanced Aging and Unique Transcriptional Activation.
Cell reports - 30 Jun 2020
Srinivasan Karpagam, Friedman Brad A, Etxeberria Ainhoa, Huntley Melanie A, van der Brug Marcel P, Foreman Oded, Paw Jonathan S, Modrusan Zora, Beach Thomas G, Serrano Geidy E, Hansen David V
Abstract excerpt
Damage-associated microglia (DAM) profiles observed in Alzheimer's disease (AD)-related mouse models reflect an activation state that could modulate AD risk or progression. To learn whether human AD microglia (HAM) display a similar profile, we develop a method for purifying cell types from frozen cerebrocortical tissues for RNA-seq analysis, allowing better transcriptome coverage than typical single-nucleus...
Topics
- Aged
- Aged, 80 and over
- Alzheimer Disease
- Animals
- Cellular Senescence
- Databases, Genetic
- Female
- Frontal Lobe
- Frozen Sections
- Gene Expression Profiling
- Genetic Predisposition to Disease
- Heterografts
- Humans