Article

Single Cell Sequencing Reveals Glial Specific Responses to Tissue Processing & Enzymatic Dissociation in Mice and Humans

2020-12-03

Abstract excerpt

A key aspect of nearly all single cell experiments is the necessity to dissociate intact tissues into single cell suspensions for processing. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that dissociation can have on ex vivo gene expression changes during this process. Microglia, the brain’s resident macrophages, are a highly dynamic population that are e...

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Identifiers and source

Literature Corpus work: 812dd102-7929-5092-95ea-013677287678
DOI: 10.1101/2020.12.03.408542

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