Article
CRISPR/Cas9-mediated gene knockout in human adipose stem/progenitor cells.
Adipocyte - 1 Dec 2020
Mandl Markus, Ritthammer Heike, Ejaz Asim, Wagner Sonja A, Hatzmann Florian M, Baumgarten Saphira, Viertler Hans P, Zwierzina Marit E, Mattesich Monika, Schiller Valerie, Rauchenwald Tina, Ploner Christian, Waldegger Petra, Pierer Gerhard, Zwerschke Werner
Abstract excerpt
The CRISPR/Cas9 system is a powerful tool to generate a specific loss-of-function phenotype by gene knockout (KO). However, this approach is challenging in primary human cells. In this technical report, we present a reliable protocol to achieve a functional KO in the genome of human adipose stem/progenitor cells (ASCs). Using Sprouty1 (SPRY1) as a model target gene for a CRISPR/Cas9 mediated KO, we particularize...
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