Fast and efficient generation of knock-in human organoids using homology-independent CRISPR/Cas9 precision genome editing

Abstract excerpt

CRISPR/Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences in human organoids awaits robust knock-in approaches. Here, we describe CRISPR /Cas9-mediated H omology-independent O rganoid T ransgenesis (CRISPR-HOT), which allows efficient generation of knock-in human organoids representing different tissues. CRISPR-HOT...

Topics

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• CRISPR-Associated Protein 9
• Deoxyribonuclease I
• DNA End-Joining Repair
• Electroporation
• Gene Knock-In Techniques
• Organoids
• Recombinational DNA Repair
• Staining and Labeling

Identifiers and source

Literature Corpus work

8f39f6d3-4bc1-5de1-a1d0-2c60d0e952a3

DOI

10.1101/2020.01.15.907766