Article

Profiling of Nascent Lariat Intermediates Reveals Key Genetic Determinants of the Timing of Human Co-transcriptional Splicing

2021-10-19

Abstract excerpt

As splicing is intimately coupled with transcription, understanding splicing mechanisms requires an understanding of splicing timing, which is currently limited. Here, we developed CoLa-seq ( co -transcriptional la riat seq uencing), a genomic assay that reports splicing timing relative to transcription through analysis of nascent lariat intermediates. In human cells, we mapped 165,282 branch points and charact...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8e5e5df1-cef6-5bd7-b39b-cbfe846c7518
DOI: 10.1101/2021.10.18.464728

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Profiling of Nascent Lariat Intermediates Reveals Key Genetic Determinants of the Timing of Human Co-transcriptional SplicingDOI 10.1101/2021.10.18.464728Read

Pervasive co-regulation of alternative splicing with transcription initiation and termination.Semantic proximity Read

Human co-transcriptional splicing kinetics and coordination revealed by direct nascent RNA sequencingSemantic proximity Read

The nascent transcriptome delineates the regulatory landscape in human health and diseaseSemantic proximity Read

Genomic sequences and RNA binding proteins predict RNA splicing kinetics in various single-cell contextsSemantic proximity Read

Transcription and splicing dynamics during early <i>Drosophila</i> developmentSemantic proximity Read

Disentangling spatial organization and splicing of rare intron classes in the human genomeSemantic proximity Read

Alternative splicing is coupled to gene expression in a subset of variably expressed genesSemantic proximity Read

Transcriptome sequencing suggests that pre-mRNA splicing counteracts widespread intronic cleavage and polyadenylationSemantic proximity Read

The competition between splicing and 3′ processing shapes the human transcriptomeSemantic proximity Read

Epigenome-based Splicing Prediction using a Recurrent Neural NetworkSemantic proximity Read

Pre-mRNA splicing is facilitated by an optimal RNA polymerase II elongation rate.Semantic proximity Read

Genomic codes governing enhancer RNA fateSemantic proximity Read