Article

Simple biochemical features underlie transcriptional activation domain diversity and dynamic, fuzzy binding to Mediator

2020-12-18

Abstract excerpt

<h4>SUMMARY</h4> Gene activator proteins comprise distinct DNA-binding and transcriptional activation domains (ADs). Because few ADs have been described, we tested domains tiling all yeast transcription factors for activation in vivo and identified 150 ADs. By mRNA display, we showed that 73% of ADs bound the Med15 subunit of Mediator, and that binding strength was correlated with activation. AD-Mediator interac...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 8dfdef88-25dc-55a0-862a-83a27ab523e7
DOI: 10.1101/2020.12.18.423551

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Simple biochemical features underlie transcriptional activation domain diversity and dynamic, fuzzy binding to MediatorDOI 10.1101/2020.12.18.423551Read

Simple biochemical features underlie transcriptional activation domain diversity and dynamic, fuzzy binding to Mediator.Semantic proximity Read

Large-scale mapping and systematic mutagenesis of human transcriptional effector domainsSemantic proximity Read

The Mediator Complex: Progress over the Past DecadeSemantic proximity Read

Functional interplay between Mediator and RSC chromatin remodeling complex controls nucleosome-depleted region maintenance at promotersSemantic proximity Read

Classification of Human Transcription Factors Based on Their Effector Domains via Unsupervised LearningSemantic proximity Read

Identification and characterization of repressive domains in <i>Drosophila</i> transcription factorsSemantic proximity Read

DNA flexibility regulates transcription factor binding to nucleosomesSemantic proximity Read

Histone modifications influence mediator interactions with chromatin.Semantic proximity Read

High-throughput discovery and characterization of human transcriptional effectorsSemantic proximity Read

GHT-SELEX demonstrates unexpectedly high intrinsic sequence specificity and complex DNA binding of many human transcription factorsSemantic proximity Read

Mediator protein mutations that selectively abolish activated transcription.Semantic proximity Read

Systematic identification of transcriptional activator domains from non-transcription factor proteins in plants and yeastSemantic proximity Read