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Article

Mediator protein mutations that selectively abolish activated transcription.

Proceedings of the National Academy of Sciences of the United States of America - 5 Jan 1999

Myers L C, Gustafsson C M, Hayashibara K C, Brown P O, Kornberg R D

Abstract excerpt

Deletion of any one of three subunits of the yeast Mediator of transcriptional regulation, Med2, Pgd1 (Hrs1), and Sin4, abolished activation by Gal4-VP16 in vitro. By contrast, other Mediator functions, stimulation of basal transcription and of TFIIH kinase activity, were unaffected. A different but overlapping Mediator subunit dependence was found for activation by Gcn4. The genetic requirements for activation...

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Identifiers

PMID: 9874773
DOI: 10.1073/pnas.96.1.67
PMCID: PMC15094

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