Article
Mediator protein mutations that selectively abolish activated transcription.
Proceedings of the National Academy of Sciences of the United States of America - 5 Jan 1999
Myers L C, Gustafsson C M, Hayashibara K C, Brown P O, Kornberg R D
Abstract excerpt
Deletion of any one of three subunits of the yeast Mediator of transcriptional regulation, Med2, Pgd1 (Hrs1), and Sin4, abolished activation by Gal4-VP16 in vitro. By contrast, other Mediator functions, stimulation of basal transcription and of TFIIH kinase activity, were unaffected. A different...
Topics
- Cell-Free System
- DNA-Binding Proteins
- Fungal Proteins
- Gene Deletion
- Gene Expression Regulation, Fungal
- Models, Genetic
- Mutation
- Protein Kinases
- Saccharomyces cerevisiae Proteins
- Structure-Activity Relationship
- Trans-Activators
- Transcription, Genetic
- Transcriptional Activation
- Yeasts