Article

Confirming Putative Variants at ≤5% Allele Frequency Using Allele Enrichment and Sanger Sequencing

2021-02-05

Abstract excerpt

Whole exome sequencing (WES) is used to identify mutations in a patient’s tumor DNA that are predictive of tumor behavior, including the likelihood of response or resistance to cancer therapy. WES has a mutation limit of detection (LoD) at variant allele frequencies (VAF) of 5%. Putative mutations called at ≤5% VAF are frequently due to sequencing errors, therefore reporting these subclonal mutations incurs risk o...

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Literature Corpus work: 3bcb2c12-3f08-5f24-bb55-11d1cac62923
DOI: 10.21203/rs.3.rs-159725/v1

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