Article

Compressed Perturb-seq: highly efficient screens for regulatory circuits using random composite perturbations

2023-01-23

Abstract excerpt

Pooled CRISPR screens with single-cell RNA-seq readout (Perturb-seq) have emerged as a key technique in functional genomics, but are limited in scale by cost and combinatorial complexity. Here, we reimagine Perturb-seq’s design through the lens of algorithms applied to random, low-dimensional observations. We present compressed Perturb-seq, which measures multiple random perturbations per cell or multiple cells pe...

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Literature Corpus work: 8ad101f2-5bab-5cb8-a025-ff57cca72b93
DOI: 10.1101/2023.01.23.525200

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