Article
Scalable, compressed phenotypic screening using pooled perturbations.
Nature biotechnology - 1 Aug 2025
Liu Nuo, Kattan Walaa E, Mead Benjamin E, Kummerlowe Conner, Cheng Thomas, Ingabire Sarah, Cheah Jaime H, Soule Christian K, Vrcic Anita, McIninch Jane K, Triana Sergio, Guzman Manuel, Dao Tyler T, Peters Joshua M, Lowder Kristen E, Crawford Lorin, Amini Ava P, Blainey Paul C, Hahn William C, Cleary Brian, Bryson Bryan, Winter Peter S, Raghavan Srivatsan, Shalek Alex K
Abstract excerpt
High-throughput phenotypic screens using biochemical perturbations and high-content readouts are constrained by limitations of scale. To address this, we establish a method of pooling exogenous perturbations followed by computational deconvolution to reduce required sample size, labor and cost. We demonstrate the increased efficiency of compressed experimental designs compared to conventional approaches through...
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