Precise base editing for the <i>in vivo</i> study of developmental signaling and human pathologies in zebrafish

Abstract excerpt

<h4>ABSTRACT</h4> While zebrafish is emerging as a new model system to study human diseases, an efficient methodology to generate precise point mutations at high efficiency is still lacking. Here we show that base editors can generate C-to-T point mutations with high efficiencies without other unwanted on-target mutations. In addition, we established a new editor variant recognizing an NAA PAM, expanding the base...

Topics

Open a Topic to create a Post that cites this publication.

• beta Catenin
• Disease Models, Animal
• Gene Editing
• Oncogenes
• Point Mutation
• Signal Transduction
• Zebrafish
• Zebrafish Proteins

Identifiers and source

Literature Corpus work

51d13113-8490-5900-a879-6f459fd18d2b

DOI

10.1101/2020.12.12.422520