Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafish

14 Jun 2022

Abstract excerpt

Precise genetic modifications in model organisms are essential for biomedical research. The recent development of PAM-less base editors makes it possible to assess the functional impact and pathogenicity of nucleotide mutations in animals. Here we first optimize SpG and SpRY systems in zebrafish by purifying protein combined with synthetically modified gRNA. SpG shows high editing efficiency at NGN PAM sites,...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR and Genetic Engineering
Share to Mosquito-borne diseases and control
Share to RNA regulation and disease

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 35701400
DOI: 10.1038/s41467-022-31034-8

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

SpG and SpRY variants expand the CRISPR toolbox for genome editing in zebrafishPMID 35701400Read ↗

Sequence Context‐Agnostic TadA‐Derived Cytosine Base Editors for Genome‐Wide Editing in ZebrafishSemantic proximity Read ↗

ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish.Semantic proximity Read ↗

TadA-Derived Cytosine Base Editor for Precise Genome Editing in ZebrafishSemantic proximity Read ↗

Precise in vivo functional analysis of DNA variants with base editing using ACEofBASEs target prediction.Semantic proximity Read ↗

Precise <i>in vivo</i> functional analysis of DNA variants with base editing using ACEofBASEs target predictionSemantic proximity Read ↗

Precise generation of bystander-free mouse models with ABE9-SpRYSemantic proximity Read ↗

Disease modeling by efficient genome editing using a near PAM-less base editor <i>in vivo</i>Semantic proximity Read ↗

Improving precision base editing of the zebrafish genome by Rad51DBD-incorporated single-base editors.Semantic proximity Read ↗

Prime editing outperforms homology-directed repair as a tool for CRISPR-mediated variant knock-in in zebrafishSemantic proximity Read ↗

Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.Semantic proximity Read ↗

Precise A•T to G•C base editing in the zebrafish genomeSemantic proximity Read ↗

Chemical modifications of adenine base editor mRNA and guide RNA expand its application scope.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.