Article

SPARP-seq reveals poly(A) tail shortening as a hallmark of the active human translatome

2025-11-26

Abstract excerpt

The poly(A) tail at the 3’ end of mRNAs plays a critical role in the synthesis of proteins, but its dynamic relationship with the translation process has been unclear. Here, using a novel single-molecule approach SPARP-seq that integrates poly(A) assessment with ribosome profiling, we demonstrate that poly(A) tail shortening is a universal hallmark of active translation in human cells. We find that most mRNAs begi...

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Identifiers and source

Literature Corpus work: 87b55247-3ec0-541b-82cc-037bc0da3de1
DOI: 10.1101/2025.11.25.690357

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