Article

Reverse Proteolysis Uncovers a Hidden Dimension of the Peptidome

2025-09-06

Abstract excerpt

Proteases are conventionally regarded as degradative enzymes, yet their catalytic machinery also permits peptide bond formation through reverse proteolysis, a process that remains poorly characterized. Here, we show that lysosomal cysteine cathepsins catalyze iterative cycles of hydrolysis and ligation to generate multi-generational fusion peptides, including hybrids derived from host-viral protein substrates. Qua...

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Identifiers and source

Literature Corpus work: 85965299-7371-57c5-8934-ed35f2b86983
DOI: 10.1101/2025.09.02.673809

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