Article

diffONT: predicting methylation-specific PCR biomarkers based on nanopore sequencing data for clinical application

2025-02-20

Abstract excerpt

DNA methylation is known to act as biomarker applicable for clinical diagnostics, especially in cancer detection. Methylation-specific PCR (MSP) is a widely used approach to screen patient samples fast and efficiently for differential methylation. During MSP, methylated regions are selectively amplified with specific primers. With nanopore sequencing, knowledge about DNA methylation is generated during direct DNA...

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Literature Corpus work: 8583da11-31ef-5081-8eac-f394d55d0a65
DOI: 10.1101/2025.02.17.638597

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