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Residual DNA impurities in AAV vectors—nature and transcription

4 Jun 2025

Abstract excerpt

Recombinant adeno-associated viruses (rAAVs) produced by transfecting DNA plasmids into mammalian cells can inadvertently package host cell DNA (hcDNA) and plasmid DNA inside their capsids. Although the percentage of these DNA impurities is low compared to the rAAV genome in vector preparations, it is essential to characterize the DNA impurities in gene therapy products due to the theoretical risks associated...

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PMID: 40599703
DOI: 10.1016/j.omtm.2025.101503

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