Article

Brr2p-mediated unwinding of U4/U6 is promoted by a mutually exclusive intra-molecular stem loop in U4 and involves destabilization of the 5’ stem-loop of U4

2025-08-02

Abstract excerpt

Before the spliceosome engages a pre-mRNA to excise its introns, the catalytic small nuclear RNA (snRNA) U6 is inactive because of base pairing with U4 snRNA; thus, spliceosome activation requires unwinding of base paired U4/U6, composed of stem I and stem II. The Ski2-like ATPase and RNA helicase Brr2p facilitates U4/U6 unwinding and the ultimately irreversible release of U4; however, the molecular mechanism behi...

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Literature Corpus work: 82cb4ad4-78cd-5c92-82b9-3b4656f3c2bb
DOI: 10.1101/2025.08.01.667943

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