Article

Barcode Fusion Genetics-Protein-fragment Complementation Assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discovery

2021-07-27

Abstract excerpt

<h4>ABSTRACT</h4> Barcode fusion genetics (BFG) utilizes deep sequencing to improve the throughput of protein-protein interaction (PPI) screening in pools. BFG has been implemented in Yeast two-hybrid (Y2H) screens (BFG-Y2H). While Y2H requires test protein pairs to localize in the nucleus for reporter reconstruction, Dihydrofolate Reductase Protein-Fragment Complementation Assay (DHFR-PCA) allows proteins to loc...

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Literature Corpus work: 7e19c285-c67b-54e9-94aa-825ce38b6936
DOI: 10.1101/2021.07.27.453987

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