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Stable Transfection of the Human Parasite <i>Leishmania major</i> Delineates a 30-Kilobase Region Sufficient for Extrachromosomal Replication and Expression

1990-03-01

Abstract excerpt

To delineate segments of the genome of the human protozoan parasite Leishmania major necessary for replication and expression, we developed a vector (pR-NEO) which can be reproducibly introduced into L. major. This DNA was derived from a 30-kilobase extrachromosomal amplified DNA bearing the dihydrofolate reductase-thymidylate synthase gene, with the coding region for neomycin phosphotransferase substituted for th...

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Identifiers and source

Literature Corpus work
7bd4f5de-085d-5076-ad65-13b9d686d079
DOI
10.1128/mcb.10.3.1084-1094.1990
Open publication

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Stable Transfection of the Human Parasite <i>Leishmania major</i> Delineates a 30-Kilobase Region Sufficient for Extrachromosomal Replication and ExpressionDOI 10.1128/mcb.10.3.1084-1094.1990
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