Homologous recombination and gene replacement at the dihydrofolate reductase-thymidylate synthase locus in Toxoplasma gondii.

doi:10.1016/0166-6851(94)90060-4

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Article

Homologous recombination and gene replacement at the dihydrofolate reductase-thymidylate synthase locus in Toxoplasma gondii.

Molecular and biochemical parasitology - 1 Feb 1994

Donald R G, Roos D S

Abstract excerpt

To investigate the feasibility of genomic transgene expression and gene targeting in Toxoplasma gondii, parasites have been transfected with constructs differing in the length of contiguous genomic sequence spanning the dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene. We have previous...

Topics

Alleles
Animals
Animals, Genetically Modified
Drug Resistance
Genes, Protozoan
Genetic Markers
Genetic Vectors
Models, Genetic
Molecular Sequence Data
Pyrimethamine
Recombination, Genetic
Sequence Homology, Nucleic Acid
Tetrahydrofolate Dehydrogenase
Thymidylate Synthase
Toxoplasma
Transformation, Genetic

Source

Open in PubMed

Identifiers

PMID: 8008022
DOI: 10.1016/0166-6851(94)90060-4

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8008022-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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