Article

Deaminase-assisted single-molecule and single-cell chromatin fiber sequencing

2024-11-06

Abstract excerpt

Gene regulation is mediated by the co-occupancy of numerous proteins along individual chromatin fibers. However, our tools for deeply profiling how proteins co-occupy individual fibers, especially at the single-cell level, remain limited. We present Deaminase-Assisted single-molecule chromatin Fiber sequencing (DAF-seq), which leverages a non-specific double-stranded DNA deaminase toxin A (SsDddA) to efficiently s...

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Literature Corpus work: 7abf54cd-4e8b-524a-b5c6-6cf078189058
DOI: 10.1101/2024.11.06.622310

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