Article

“Ancestralization” of human pluripotent stem cells by multiplexed precise genome editing

2018-06-08

Abstract excerpt

We show that inactivation of the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) results in a drastic increase in efficiency of precise genome editing with CRISPR enzymes in human stem cells, allowing up to 79% of chromosomes to carry an intended nucleotide substitution when a single genomic site is targeted. When three different genes are simultaneously targeted, 12% of the isolated cells carry the targ...

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Literature Corpus work: 78c8a9ab-353b-5968-ba53-d2f8f0596b1e
DOI: 10.1101/342311

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