Article
Genome-scale exon perturbation screens uncover exons critical for cell fitness.
Molecular cell - 11 Jul 2024
Xiao Mei-Sheng, Damodaran Arun Prasath, Kumari Bandana, Dickson Ethan, Xing Kun, On Tyler A, Parab Nikhil, King Helen E, Perez Alexendar R, Guiblet Wilfried M, Duncan Gerard, Che Anney, Chari Raj, Andresson Thorkell, Vidigal Joana A, Weatheritt Robert J, Aregger Michael, Gonatopoulos-Pournatzis Thomas
Abstract excerpt
CRISPR-Cas technology has transformed functional genomics, yet understanding of how individual exons differentially shape cellular phenotypes remains limited. Here, we optimized and conducted massively parallel exon deletion and splice-site mutation screens in human cell lines to identify exons that regulate cellular fitness. Fitness-promoting exons are prevalent in essential and highly expressed genes and...
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