Article

Highly selective Cooperative Primers for multiplex detection of rare mutations

2021-07-23

Abstract excerpt

We describe a real time PCR-based technique capable of detecting and quantifying rare somatic mutations in circulating tumor DNA reference materials. Our approach utilizes previously described Cooperative Primers, structurally modified to exhibit high allele-specificity and one-copy target sensitivity. Cooperative Primers are bi-functional molecules, consisting of a high affinity probe fragment that guarantees sen...

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Identifiers and source

Literature Corpus work: 76619194-4655-55cd-98ed-27de6fc0e346
DOI: 10.1101/2021.07.23.453558

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