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Article

Single-Color Digital PCR Provides High-Performance Detection of Cancer Mutations from Circulating DNA.

The Journal of molecular diagnostics : JMD - 1 Sept 2017

Wood-Bouwens Christina, Lau Billy T, Handy Christine M, Lee HoJoon, Ji Hanlee P

Abstract excerpt

We describe a single-color digital PCR assay that detects and quantifies cancer mutations directly from circulating DNA collected from the plasma of cancer patients. This approach relies on a double-stranded DNA intercalator dye and paired allele-specific DNA primer sets to determine an absolute count of both the mutation and wild-type-bearing DNA molecules present in the sample. The cell-free DNA assay uses an...

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Identifiers

PMID: 28818432
DOI: 10.1016/j.jmoldx.2017.05.003
PMCID: PMC6593258

Genes

BRAF
KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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