Article

Transcript-Specific Site-Directed RNA Editing Reveals Principles for Splice-aware guide RNA Design

2026-07-30

Abstract excerpt

<h4>ABSTRACT</h4> Site-directed RNA editing (SDRE) utilizing the adenosine deaminase acting on RNA (ADAR) enzymes is commonly facilitated by guide RNAs (gRNAs) optimized to enhance on-target editing and minimize bystander effects. However, the impact of gRNA binding and ADAR-mediated SDRE on canonical pre-mRNA splicing remains poorly understood. Here, we developed an in vitro transcript-specific editing strategy...

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Literature Corpus work: 6b161d09-87e3-5f46-8eab-5542c7c47306
DOI: 10.64898/2026.07.30.741163

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