Article

BASELINE: A CRISPR Base Editing Platform for Mammalian-Scale Single-Cell Lineage Tracing

2025-03-20

Abstract excerpt

A cell’s fate is shaped by its inherited state, or lineage, and the ever-shifting context of its environment. CRISPR-based recording technologies are a promising solution to map the lineage of a developing system, yet challenges remain regarding single-cell recovery, engineering complexity, and scale. Here, we introduce BASELINE, which uses base editing to generate high-resolution lineage trees in conjunction with...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 62decd72-8d90-553d-a42d-2baed180a1d7
DOI: 10.1101/2025.03.19.644238

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

BASELINE: A CRISPR Base Editing Platform for Mammalian-Scale Single-Cell Lineage TracingDOI 10.1101/2025.03.19.644238Read

BASELINE: a CRISPR base editing platform for mammalian-scale single-cell lineage tracingSemantic proximity Read

Whole-organism lineage tracing by combinatorial and cumulative genome editing.Semantic proximity Read

Assessing the inference of single-cell phylogenies and population dynamics from CRISPR lineage recordingsSemantic proximity Read

Charting Single Cell Lineage Dynamics and Mutation Networks via Homing CRISPRSemantic proximity Read

Regenerative base editing enables deep lineage recordingSemantic proximity Read

DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsSemantic proximity Read

Inference of Single-Cell Phylogenies from Lineage Tracing DataSemantic proximity Read

Lineage tracing and analog recording in mammalian cells by single-site DNA writing.Semantic proximity Read

Inference of single-cell phylogenies from lineage tracing data using Cassiopeia.Semantic proximity Read

Strategies for resolving cellular phylogenies from sequential lineage tracing data.Semantic proximity Read

Simulation of CRISPR-Cas9 editing on evolving barcode and accuracy of lineage tracing.Semantic proximity Read

Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elementsSemantic proximity Read