Article

Brief Freezing Steps Lead to Robust Immunofluorescence in <i>Drosophila</i> Larval Brains

2018-05-13

Abstract excerpt

Drosophila melanogaster possess complex neuronal networks regulating sophisticated behavioral outputs that aid in studying the molecular mechanisms of neuronal function and neurodegenerative disease. Immunofluorescence (IF) techniques provide a way to visualize the spatiotemporal organization of these networks, permitting observation of their development, functional location, remodeling, and eventually - degradati...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 5c809bcf-d63e-558e-b76f-836bd68c9494
DOI: 10.1101/319913

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Brief Freezing Steps Lead to Robust Immunofluorescence in <i>Drosophila</i> Larval BrainsDOI 10.1101/319913Read

Cryo-FIB workflow for imaging brain tissue via <i>in situ</i> cryo-electron microscopySemantic proximity Read

Ultrafast tissue staining with chemical tagsSemantic proximity Read

ADAPT-3D: Accelerated Deep Adaptable Processing of Tissue for 3-Dimensional Fluorescence Tissue Imaging for Research and Clinical SettingsSemantic proximity Read

Whole-mount immunostaining of Drosophila skeletal muscle.Semantic proximity Read

Effective Rapid Blood Perfusion in <i>Xenopus</i>Semantic proximity Read

Simple and Rapid Tissue Clearing Method for Three-Dimensional Histology of the Pancreas.Semantic proximity Read

Single-cell phenotyping within transparent intact tissue through whole-body clearing.Semantic proximity Read

A method for single molecule localization microscopy of tissues reveals non-random distribution of nuclear pores in <i>Drosophila</i>Semantic proximity Read

Aldehyde-based cryopreservation of whole brainsSemantic proximity Read

CryoFISH: fluorescence in situ hybridization on ultrathin cryosections.Semantic proximity Read

Neuronal quantification in the primary motor cortex of mouse brains fixed with solutions from human gross anatomy laboratoriesSemantic proximity Read

Alternative Method to Detect Neuronal Degeneration and Amyloid β Accumulation in Free-Floating Brain Sections With Fluoro-Jade.Semantic proximity Read