Article
CryoFISH: fluorescence in situ hybridization on ultrathin cryosections.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2010
Xie Sheila Q, Lavitas Liron-Mark, Pombo Ana
Abstract excerpt
The visualization of cellular structures and components has become an invaluable tool in biological and medical sciences. Imaging subcellular compartments and single molecules within a cell has prompted the development of a wide range of sample preparation techniques as well as various microscope devices to obtain images with increased spatial resolution. Here, we present cryoFISH, a method for fluorescence in...
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