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A method for single molecule localization microscopy of tissues reveals non-random distribution of nuclear pores in <i>Drosophila</i>

2021-05-25

Abstract excerpt

Single Molecule Localisation Microscopy (SMLM) can provide nanoscale resolution in thin samples but has rarely been applied to tissues, because of high background from out of focus emitters. Here we describe a line scanning microscope that provides optical sectioning for SMLM in tissues. Imaging endogenously-tagged nucleoporins and F-actin on this system using DNA- and peptide-PAINT routinely gives 30nm resolution...

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Literature Corpus work
bfddcbee-9e0c-5f2f-976d-d65b13abec36
DOI
10.1101/2021.05.24.445468
Open publication

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A method for single molecule localization microscopy of tissues reveals non-random distribution of nuclear pores in <i>Drosophila</i>DOI 10.1101/2021.05.24.445468
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