Article
Whole-mount immunostaining of Drosophila skeletal muscle.
Nature protocols - 1 Dec 2013
Hunt Liam C, Demontis Fabio
Abstract excerpt
Skeletal muscle undergoes marked functional decay during aging in humans, but the cell biological mechanisms responsible for this process are only partly known. Age-related muscle dysfunction is also a feature of aging in the fruit fly Drosophila melanogaster. Here we describe a detailed step-by-step protocol, which takes place over 3 d, for whole-mount immunostaining of Drosophila flight muscle. The skeletal...
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