Article

Cryo-FIB workflow for imaging brain tissue via <i>in situ</i> cryo-electron microscopy

2023-02-12

Abstract excerpt

Cryo-electron microscopy (cryo-EM) enables the study of protein complexes, cytoskeletal elements, and organelles in three dimensions without the use of chemical fixation. Most cryo-EM studies focus on vitreously frozen individual cells separated from their native tissue contexts. This reliance on imaging of single cells is primarily due to technical challenges associated with preparing fresh tissue sections at a t...

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Literature Corpus work: cf191c0b-c8a5-5636-8ea7-e6692abd6647
DOI: 10.1101/2023.02.11.528064

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