A step by step protocol for stabilizing specific DNA G-quadruplexes by CRISPR guided G-quadruplex binding proteins and ligands

Abstract excerpt

<title>Abstract</title> <p>Despite the importance of DNA G-quadruplexes (G4s) in health and disease being demonstrated, technologies to readily manipulate specific G4 folding for functional analysis and therapeutic purposes are lacking. Here, we employ the G4-stabilizing protein/ligand in conjunction with CRISPR system to selectively facilitate single or multiple targeted G4 folding within specific genomic loci....

Topics

Open a Topic to create a Post that cites this publication.

• CDC2 Protein Kinase
• Cell Cycle Checkpoints
• CRISPR-Cas Systems
• DNA Repair
• G-Quadruplexes
• Gene Editing
• Mutation
• Thymidine Kinase

Identifiers and source

Literature Corpus work

59368814-5eac-55e0-80f6-38e923163f44

DOI

10.21203/rs.3.pex-2648/v1