Article

AsaruSim: a single-cell and spatial RNA-Seq Nanopore long-reads simulation workflow

2024-09-24

Abstract excerpt

<h4>Motivation</h4> The combination of long-read sequencing technologies like Oxford Nanopore with single-cell RNA sequencing (scRNAseq) assays enables the detailed exploration of transcriptomic complexity, including isoform detection and quantification, by capturing full-length cDNAs. However, challenges remain, including the lack of advanced simulation tools that can effectively mimic the unique complexities of...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 55b10c0e-a164-5980-b7dd-14f35aa7209f
DOI: 10.1101/2024.09.20.613625

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

AsaruSim: a single-cell and spatial RNA-Seq Nanopore long-reads simulation workflowDOI 10.1101/2024.09.20.613625Read

Scywalker: scalable end-to-end data analysis workflow for nanopore single-cell transcriptome sequencingSemantic proximity Read

A systematic benchmark of Nanopore long read RNA sequencing for transcript level analysis in human cell linesSemantic proximity Read

SCOTCH: isoform-level characterization of gene expression through long-read single-cell RNA sequencingSemantic proximity Read

Nanopore native RNA sequencing of a human poly(A) transcriptomeSemantic proximity Read

High throughput single cell long-read sequencing analyses of same-cell genotypes and phenotypes in human tumors.Semantic proximity Read

R2C2: Improving nanopore read accuracy enables the sequencing of highly-multiplexed full-length single-cell cDNASemantic proximity Read

Trans-NanoSim characterizes and simulates nanopore RNA-seq dataSemantic proximity Read

Benchmarking Next-Generation Sequencing Platforms: A Comprehensive Comparison Of Single-Cell RNA-Seq from Ultima UG 100 vs. Illumina NovaSeq X PlusSemantic proximity Read

NanoTrans: an integrated computational framework for comprehensive transcriptome analysis with Nanopore direct RNA sequencingSemantic proximity Read

Decoding the human PBMC isonome: Isoform-level resolution with single-cell long-read transcriptomicsSemantic proximity Read

High throughput detection of variation in single-cell whole transcriptome through streamlined scFAST-seqSemantic proximity Read

Comprehensive characterization of single cell full-length isoforms in human and mouse with long-read sequencingSemantic proximity Read