Article

Decoding the human PBMC isonome: Isoform-level resolution with single-cell long-read transcriptomics

2025-10-17

Abstract excerpt

Long-read single-cell RNA sequencing provides an opportunity to understand human health and disease at a level difficult to resolve with bulk or short-read methods. This approach enables isoform-level investigation of cellular diversity and disease mechanisms and definition of cell-types, rather than using genes alone. Using a modified, microfluidic-free PIPseq workflow and computational pipeline adapted for Oxfor...

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Literature Corpus work: a0a7cc62-1f3e-572f-9ff0-96b0d8f4170a
DOI: 10.1101/2025.10.16.682832

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