Article

CRISPR/Cas9 interrogation of the mouse <i>Pcdhg</i> gene cluster reveals a crucial isoform-specific role for <i>Pcdhgc4</i>

2019-08-19

Abstract excerpt

<h4>ABSTRACT</h4> The mammalian Pcdhg gene cluster encodes a family of 22 cell adhesion molecules, the gamma-Protocadherins (γ-Pcdhs), critical for neuronal survival and neural circuit formation. The extent to which isoform diversity–aγ-Pcdh hallmark–is required for their functions remains unclear. We used a CRISPR/Cas9 approach to reduce isoform diversity, targeting each Pcdhg variable exon with pooled sgRNAs...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 540ac4a4-b873-5ef2-ade9-512d73e72745
DOI: 10.1101/739508

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPR/Cas9 interrogation of the mouse <i>Pcdhg</i> gene cluster reveals a crucial isoform-specific role for <i>Pcdhgc4</i>DOI 10.1101/739508Read

CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4.Semantic proximity Read

Protocadherin γC4 regulates neuronal survival and dendritic self-avoidanceSemantic proximity Read

Protocadherin γC4 regulates neuronal survival and dendritic self-avoidance.Semantic proximity Read

The role and expression of the protocadherin-alpha clusters in the CNS.Semantic proximity Read

Combinatorial Effects of Alpha- and Gamma-Protocadherins on Neuronal Survival and Dendritic Self-Avoidance.Semantic proximity Read

A new mouse mutant with a discrete mutation in Pcdhgc5 reveals that the Protocadherin γC5 isoform is not essential for dendrite arborization in the cerebral cortex.Semantic proximity Read

Cellular and behavioral characterization of <i>Pcdh19</i> mutant mice: subtle molecular changes, increased exploratory behavior and an impact of social environmentSemantic proximity Read

Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.Semantic proximity Read

Functional analysis of the epilepsy gene <i>Pcdh19</i> using a novel conditional GFP-reporter mouse modelSemantic proximity Read

Expansion of stochastic expression repertoire by tandem duplication in mouse Protocadherin-α cluster.Semantic proximity Read

Impaired migration and premature differentiation underlie the neurological phenotype associated with PCDH12 loss of functionSemantic proximity Read

Differential Expression of α, β, and γ Protocadherin Isoforms During Differentiation, Aging, and CancerSemantic proximity Read