Article
CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4.
PLoS genetics - 1 Dec 2019
Garrett Andrew M, Bosch Peter J, Steffen David M, Fuller Leah C, Marcucci Charles G, Koch Alexis A, Bais Preeti, Weiner Joshua A, Burgess Robert W
Abstract excerpt
The mammalian Pcdhg gene cluster encodes a family of 22 cell adhesion molecules, the gamma-Protocadherins (γ-Pcdhs), critical for neuronal survival and neural circuit formation. The extent to which isoform diversity-a γ-Pcdh hallmark-is required for their functions remains unclear. We used a CRISPR/Cas9 approach to reduce isoform diversity, targeting each Pcdhg variable exon with pooled sgRNAs to generate an...
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