Article

A novel RNA-binding activity of ECD contributes to U5 snRNP stability and pre-mRNA splicing

2025-01-27

Abstract excerpt

Human ecdysoneless protein (ECD) plays an essential role in regulating cell cycle progression and cell survival. ECD has previously been implicated in RNA splicing through its association with spliceosomal proteins. Here, using EMSA, fluorescence polarization assays, and mutational analysis, we demonstrate that ECD directly binds to RNA. Enhanced CLIP-seq analysis identified a broad repertoire of mRNAs bound to EC...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 528a4d89-dcc6-5fba-850f-d8ca6cf420f1
DOI: 10.1101/2025.01.24.634785

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A novel RNA-binding activity of ECD contributes to U5 snRNP stability and pre-mRNA splicingDOI 10.1101/2025.01.24.634785Read

Ecd promotes U5 snRNP maturation and Prp8 stability.Semantic proximity Read

Ecdysoneless Protein Regulates Viral and Cellular mRNA Splicing to Promote Cervical OncogenesisSemantic proximity Read

NRDE2 Interacts with an Early Transcription Elongation Complex and Widely Impacts Gene ExpressionSemantic proximity Read

Exon junction complex-associated multi-adapter RNPS1 nucleates splicing regulatory complexes to maintain transcriptome surveillanceSemantic proximity Read

Unexpected role of the steroid-deficiency protein ecdysoneless in pre-mRNA splicing.Semantic proximity Read

Rapid UPF1 depletion illuminates the temporal dynamics of the NMD-regulated transcriptome in human cellsSemantic proximity Read

FRG1 Regulates Nonsense-Mediated mRNA Decay by Modulating UPF1 LevelsSemantic proximity Read

Exonuclease assisted mapping of protein-RNA interactions (ePRINT)Semantic proximity Read

Depletion of the RNA-binding protein PURA triggers changes in posttranscriptional gene regulation and loss of P-bodiesSemantic proximity Read

Resources for the comprehensive discovery of functional RNA elementsSemantic proximity Read

Comprehensive Identification of RNA-Binding Domains in Human Cells.Semantic proximity Read

The EDC4-XRN1 axis controls P-body dynamics to link mRNA decapping with decaySemantic proximity Read