Article

Protein language models-assisted engineering of Uracil-N glycosylase enables programmable T-to-G and T-to-C base editing

2023-10-29

Abstract excerpt

Current base editors use DNA deamination enzymes, such as cytidine deaminase (CBE) or adenine deaminase (ABE), to facilitate transition nucleotide substitutions. Combining CBE or ABE with glycosylase enzymes, including CGBE and AYBE, can induce limited transversion mutations. Nonetheless, a critical demand remains for base editors capable of generating alternative mutation types, such as T>G corrections, which cou...

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Literature Corpus work: 4a865585-5a71-52ad-b3c4-5d58ac7e18d1
DOI: 10.1101/2023.10.29.564583

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