Article

A Single-Molecule Long-Read Survey of Human Transcriptomes using LoopSeq Synthetic Long Read Sequencing

2019-01-28

Abstract excerpt

State-of-the-art short-read transcriptome sequencing methods employ unique molecular identifier (UMI) to accurately classify and count mRNA transcripts. A fundamental limitation of UMI-based short-read transcriptome sequencing is that each read typically covers a small fraction of the transcript sequence. Efforts to accurately characterize splicing isoforms, arguably the largest source of variation in Human gene e...

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Literature Corpus work: 488292ba-ecb7-5059-9b40-e1d15ff3ef43
DOI: 10.1101/532135

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