Article
Annotation-free quantification of RNA splicing using LeafCutter.
Nature genetics - 1 Jan 2018
Li Yang I, Knowles David A, Humphrey Jack, Barbeira Alvaro N, Dickinson Scott P, Im Hae Kyung, Pritchard Jonathan K
Abstract excerpt
The excision of introns from pre-mRNA is an essential step in mRNA processing. We developed LeafCutter to study sample and population variation in intron splicing. LeafCutter identifies variable splicing events from short-read RNA-seq data and finds events of high complexity. Our approach obviates the need for transcript annotations and circumvents the challenges in estimating relative isoform or exon usage in...
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